منابع مشابه
Embryonic Chick Intestine in Organ Culture
Duodena from 20-day-old chick embryos can be maintained in large scale organ culture on specially designed stainless-steel grids in contact with serum-free medium for 48 h with excellent preservation of mucosal structure at both the light and electron microscope levels. Although mitotic rate was subnormal, several other factors attest to the essential viability of the cultured intestine: L-leuc...
متن کاملEMBRYONIC CHICK INTESTINE IN ORGAN CULTURE A Unique System for the Study of the Intestinal Calcium Absorptive Mechanism
Duodena from 20-day-old chick embryos can be maintained in large scale organ culture on specially designed stainless-steel grids in contact with serum-free medium for 48 h with excellent preservation of mucosal structure at both the light and electron microscope levels . Although mitotic rate was subnormal, several other factors attest to the essential viability of the cultured intestine : L-le...
متن کاملEffects of cytochalasin B on the formation of previllous ridges and the appearance of long microvillous-like processes in the organ culture system of chick embryonic intestine.
Between 8 and 12 days of incubation the embryonic duodenum serially constructs with relative regularity the previllous ridges upon which the definitive villi later form. The effects of cytochalasin B (CB) on the formation of these previllous ridges of the duodena of developing chick embryos were studied, varying the concentrations and exposure time of CB in the organ culture system. The results...
متن کاملDevelopment of Fetal Monolayer Culture Rat Intestine in Organ and
Maturation and differentiation of intestinal epithelial cells was demonstrated in segments of fetal rat small intestine, maintained for more than a month in suspension organ culture, by ultrastructural, biochemical, and immunological criteria. Over a 5-7 d period, fragments of fetal intestine evolved into globular structures covered with a single columnar epithelium ultrastructurally similar to...
متن کاملDevelopment of fetal rat intestine in organ and monolayer culture
Maturation and differentiation of intestinal epithelial cells was demonstrated in segments of fetal rat small intestine, maintained for more than a month in suspension organ culture, by ultrastructural, biochemical, and immunological criteria. Over a 5-7 d period, fragments of fetal intestine evolved into globular structures covered with a single columnar epithelium ultrastructurally similar to...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1973
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.58.1.64